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GERBU Biotechnik GmbH
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Merck KGaA
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BioShop
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Biowest SAS
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Image Search Results
Journal: Bio-protocol
Article Title: Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells
doi: 10.21769/BioProtoc.3678
Figure Lengend Snippet: Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope (10x objective).
Article Snippet: Micro cover glasses, round, No. 1, 18 mm (VWR ® International, catalog number: CA-48380-046), sterilize coverglasses using autoclave Microscope slides (Fisherbrand, Fisher Scientific, catalog number: 12-550-15) Clear tissue culture-treated 12-well microplates, (Corning Costar ® , Millipore-Sigma, catalog number: CLS3513) Parafilm Paper towel Cell line: LLC-PK 1 kidney tubule epithelial cell line (European Collection of Authenticated Cell Cultures (Salisbury, UK), catalog number: 86121112) For cell culture: Dulbecco's Modified Eagle Medium (D-MEM), low glucose, with L-glutamine 110 mg/L sodium pyruvate (Thermo Fisher, Gibco, catalog number: 11885084) 10% Fetal Bovine Serum (FBS) (Thermo Fisher, Gibco, catalog number: 12483-020) 1% Penicillin-Streptomycin (Penicillin-Streptomycin, 100x, sterile-filtered, cell culture tested) (Millipore-Sigma Aldrich, catalog number: P-4333) Trypsin-EDTA (0.05% Trypsin with EDTA 4Na) (Thermo Fisher, Gibco, catalog number: 25300062) Phosphate buffered saline (PBS) (Thermo Fisher, Gibco, catalog number: 10010023) Methanol (BioShop Canada, catalog number: Met302), cool to -20 °C before using
Techniques: Microscopy